This page stores the instruction manuals for the molecular biology kits that are used in our lab.
Our general lab protocol for PCR, gel extraction, and cloning [.pdf] (in Chinese)
Section 1. DNA/RNA Purification
Plasmid prep
GMbiolab Plasmid Miniprep Kit [.pdf]
QIAfilter Plasmid MIDI Prep Kit [.pdf]
ZymoResearch ZR Plasmid Kit [.pdf]
Genomic DNA extraction
ZymoResearch ZR Genomic DNA-Tissue Microprep [.pdf]
ZymoResearch ZR Genomic DNA-Tissue Miniprep [.pdf]
DNA gel extraction or reaction cleanup
ZymoResearch DNA Clean & Concentrator-5 [.pdf]
ZymoResearch Zymoclean Gel DNA Recovery Kit [.pdf]RNA extraction
Ambion TRIzol [.pdf]
Ambion PureLink RNA Miniprep Kit [.pdf]
ZymoResearch Direct-zol RNA MiniPrep Kit [.pdf]
ZymoResearch Quick-RNA MiniPrep [.pdf]
mRNA purification
Thermo MagJET mRNA Enrichment Kit [.pdf]
RNA reaction cleanup
ZymoResearch RNA Clean & Concentrator-5 [.pdf]
ZymoResearch RNA Clean & Concentrator-25 [.pdf]
Section 2. DNA/RNA Polymerases
DNA polymerase
Finnzyme Phusion High-Fidelity DNA Polymerase [.pdf]
NEB Klenow Fragment [.html]
Thermo Terminal Transferase [.pdf]
RNA polymerase
SP6/T7/T3 RNA Polymerase: refer to our standard probe synthesis protocol for probe synthesis [.html]; for synthesizing unlabeled RNA, simply substitute the RNA labeling mix with 40 mM NTP solution (10 mM for each nucleotide).
Long dsRNA synthesis
MEGAscript T7/SP6 Kit [.pdf]
CRISPR gRNA & shRNA synthesis
MEGAshortscript T7 Kit [.pdf]In vitro transcription of mRNA
mMESSAGE mMACHINE SP6 Kit [.pdf]
Poly(A) Tailing Kit [.pdf]
Reverse transcription
Invitrogen SuperScript III First-Strand Synthesis System [.pdf]
Thermo RevertAid H Minus First Strand cDNA Synthesis Kit [.pdf]
Section 3. Cloning
Ambion FirstChoice RLM RACE Kit [.pdf]
Invitrogen pCRII Dual Promoter Vector TA Cloning Kit [.pdf]
Invitrogen Champion pET102 Directional TOPO Expression Kit [.pdf]
Promega pGEM-T and pGEM-T Easy Vector System [.pdf]
Thermo CloneJET PCR Cloning Kit [.pdf]
Yeastern ECOS Competent Cells [.pdf]
Section 4. Western Blot
Invitrogen Mini Gel Tank [.pdf], quick reference card [.pdf], for Bolt mini Gel [.html]
Mini Gel Tank adaptation for Novex [.html] and NuPAGE [.html] Gels [.pdf];
Invitrogen iBlot 2 Dry Blotting System [.pdf], quick reference card [.pdf]
Invitrogen iBind Western Device [.pdf], quick referebce card [.pdf]
SuperSignal West Femto Substrate Kit [.pdf]