Protocols

 


 

Leech genome resources


 

Utility plasmids (available upon request)

plasmid ID
description and version history

 

pBSMNEF1P [.ape]

For driving transient transient gene expression.
Helobdella EF1α promoter - MCS - SV40 polyadenylation sequence cassett flanked by meganuclease sites. Built by Alberto Stolfi [at UC Berkeley, 2007].


 

pMiEF1P-SV40 v.1 [.ape]

For genome insertion of transgene.
Helobdella
EF1α promoter - MCS - SV40 polyadenylation sequence cassett flanked by minos transposable elements. Built by Alex Hartenstein and D.-H. Kuo [at UC Berkeley, 2012].

pMiEF1P-SV40 v.1.1 [.ape]

Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014].

pMiEF1P-SV40 v.1.2 [.ape]

Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014].

pMiEF1P-SV40 v.1.3 [.ape]

Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014].


 

pTol2EF1P [.ape]

For genome insertion of transgene.
Helobdella EF1α promoter - MCS - SV40 polyadenylation sequence cassett flanked by Tol2 transposable elements. Built by D.-H. Kuo [at NTU, 2015].


 

pEF1P nCas9n

For transiently expressing nCas9n in Helobdella. Built on pBSMNEF1P backbone by D.-H. Kuo [at NTU, 2015].

pEF1P nCas9n(D10A)

For transiently expressing nCas9n(D10A) nickase in Helobdella. Built on pBSMNEF1P backbone by D.-H. Kuo [at NTU, 2015].