Leech genome resources
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Helobdella robusta (JGI)
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Helobdella robusta (Ensembl)
Utility plasmids (available upon request)
plasmid ID |
description and version history |
pBSMNEF1P [.ape] |
For driving transient transient gene expression. |
pMiEF1P-SV40 v.1 [.ape] |
For genome insertion of transgene. |
pMiEF1P-SV40 v.1.1 [.ape] |
Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014]. |
pMiEF1P-SV40 v.1.2 [.ape] |
Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014]. |
pMiEF1P-SV40 v.1.3 [.ape] |
Restriction site modifications in pMiEF1P. Made by D.-H. Kuo [at NTU, 2014]. |
pTol2EF1P [.ape] |
For genome insertion of transgene. |
pEF1P nCas9n |
For transiently expressing nCas9n in Helobdella. Built on pBSMNEF1P backbone by D.-H. Kuo [at NTU, 2015]. |
pEF1P nCas9n(D10A) |
For transiently expressing nCas9n(D10A) nickase in Helobdella. Built on pBSMNEF1P backbone by D.-H. Kuo [at NTU, 2015]. |
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The Kuo lab reagent database
(For internal access only, account and password are required.)