1. Chen, Y. H., C. T. Liang and H. J. Tsai* 2002. Expression, purification and DNA-binding activity of tilapia muscle-specific transcription factor MyoD produced by Escherichia coli. Comp. Biochem. Physiol., 131B: 795-805. (SCI Journal)

2. Chen, Y. H. and H. J. Tsai* 2002. Treatment with Myf5-morpholino results in somite patterning and brain formation defects in zebrafish. Differentiation, 70: 447-456. (SCI Journal)

3. Ho, Y. L., Y. H. Shau, H. J. Tsai, L. C. Lin, P. J. Huang and F. J. Hsieh* 2002. Assessment of zebrafish cardiac performance using doppler echocardiography and power angiography. Ultrasound Med. Biol., 28: 1137-1143. (SCI Journal)

4. Wang, T. M., Y. H. Chen, C. F. Liu and H. J. Tsai* 2002. Functional analysis of the proximal promoter region of fish rhodopsin and Myf-5 genes using transgenesis. Marine Biotechnol., 4: 247-255. (SCI Journal)

5. Hsiao, C. D., W. Y. Tsai and H. J. Tsai* 2002. Isolation and expression of two zebrafish homologous of parvalbmin genes related to chicken CPV3 and mammalian oncomodulin. Mech. Dev., 119S:S161-S166. (SCI Journal)

6. Hsiao, C. D., W. Y. Tsai, L. S. Horng and H. J. Tsai* 2003. Molecular structure and developmental expression of three muscle-type of troponin T genes in zebrafish. Dev. Dynamics, 227:266-279. (SCI Journal)

7. Huang, C. J., C. T. Tu, C. D. Hsiao, F. J. Hsieh and H. J. Tsai* 2003. Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish. Dev. Dynamics, 228:30-40. (SCI Journal)

8. Chen, Y. H., H. C. Lee, C. F. Liu, C. Y. Lin and H. J. Tsai* 2003. Novel regulatory sequence -82/-62 functions as a key element to drive the somite-specificity of zebrafish myf-5. Dev. Dynamics, 228:41-50. (SCI Journal)

9. Hsiao, C. D. and H. J. Tsai* 2003. Transgenic zebrafish with fluorescent germ cells: a useful tool to visualize germ cells proliferation and juvenile hermaphroditism in vivo. Dev. Biol., 262:313-323. (SCI Journal)

10. Raya, A., C. M. Koth, D. Buscher, Y. Kawakami, T. Itoh, R. M. Raya, G. Sternik, H. J. Tsai, C. Rodriguez-Esteban and J. C, Izpisua-Belmonte* 2003. Activation of Notch signaling pathway precedes heart regeneration in zebrafish. Proc. Natl. Acad. Sci. U.S.A.,100: 11889-11895. (SCI Journal)

11. Shu, X., K. Cheng, N. Pate, F. Chen, E. Joseph, H. J. Tsai and J. Chen*. 2003. Na, K-ATPase is essential for embryonic heart development in the zebrafish. Development, 130:6165-6173. (SCI Journal)

12. Hsiao, C. D., M. Ekker and H. J. Tsai* 2003. Skin-specific expression of ictacalcin, a homolog of the S100 genes during zebrafish embryogenesis. Dev. Dynamics, 228:745–750. (SCI Journal)

13. Chu, S. W., T. M. Liu, C. K. Sun,* C. Y. Lin and H. J. Tsai. 2003. Real-time second- harmonic- generation microscopy based on a 2-GHz repetition rate Ti:sapphire Laser. Optics Express., 11:933-938. (SCI Journal)

14. Chu, S. W., S. Y. Chen, T. H. Tsai, T. M. Liu, C.Y. Lin, H. J. Tsai and C. -K. Sun.* 2003. In vivo developmental biology study using noninvasive multi-harmonic generation microscopy. Optics Express., 11:3093-3099. (SCI Journal)

15. Tsai, H. J. * 2003. Transgenic fish: research and application. J. Fish. Soc. Taiwan, 30:263-277 (invited review article)

16. Sun, C. K.,* S. -W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C. -Y. Lin and H. J. Tsai. 2004. Higher harmonic generation microscopy for developmental biology. J. Struct. Biol., 147: 19-30 (SCI Journal)

17. Lin, C. Y., Y. H. Chen, H. C. Lee and H. J. Tsai*. 2004. Novel cis-element in intron 1 represses somite expression of zebrafish myf-5. Gene, 334:63-72. (SCI Journal)

18. Huang, C. J., T. S. Jou, Y. L. Ho, W. H. Lee, Y. T. Jeng, F. J. Hsieh and H. J. Tsai*. 2005. Conditional expression of a myocardium-specific transgene in zebrafish transgenic lines. Dev. Dynamics, 233:1294-1303 (SCI Journal)

19. Wang, Y. H., Y. H. Chen, J. H. Lu and H. J. Tsai*. 2005. A 23-amino-acid residue spanning the basic domain targets zebrafish myogenic regulatory factor Myf-5 into nucleolus. DNA Cell Biol. 24: 651-660. (SCI Journal)

20. Chen, H. L., H. S. Yang, R. Huang and H. J. Tsai*. 2006. Transfer of a foreign gene to Japanese abalone (Haliotis diversicolor supertexta) by direct testis-injection. Aquaculture, 253:249-258. (SCI Journal)

21. Lee, H. C., H. Y. Huang, C. Y. Lin, Y. H. Chen and H. J. Tsai*. 2006. Foxd3 mediates zebrafish myf5 expression during early somitogenesis Dev. Biol. 290:359-372 (SCI journal)

22. Wang, Y. H., Y. H. Chen, Y. J. Lin and H. J. Tsai*. 2006. Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line. Mech. Dev.:Gene Expression Patterns 6:335-339. (SCI journal)

23. Tsai, T. H., C. Y. Lin, H. J. Tsai, S. Y. Chen, S. P. Tai, K. H. Lin and C. K. Sun*. 2006. Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section. Opt. Lett. 31: 930-932. (SCI Journal)

24. Wang, Y. H., Y. H. Chen, J. H. Lu, Y. J. Lin, M. Y. Chang and H. J. Tsai*. 2006. Epidermis-restricted expression of zebrafish cytokeratin II is controlled by a -141/+85 minimal promoter, and cassette -141/-111 is esential for driving the tissue-specificity. Differentiation 74:186-194. (SCI journal)

25. Wang, Y. H., Y. H. Chen, T. N. Wu and H. J. Tsai*. 2006. A keratin 18 transgenic zebrafish Tg(k18(2.9):RFP) treated with inorganic arsenite reveals visible overproliferation of epithelial cells. Toxicol. Lett. 163:191-197. (SCI journal)

26. Sato, M., H. J. Tsai and H. J. Yost*. 2006. Semaphorin3D regulates invasion of cardiac neural crest cells into the primary heart field. Dev. Biol. 298: 12-21. (SCI Journal)

27. Chun, C. Z., H. J. Tsai and T. T. Chen*. 2006. Trout Ea4- or Human Eb-Peptide of Pro-IGF-I disrupts heart, red blood cell, and vasculature development in zebrafish embryos. Mol. Reprod. Dev. 73:1112–1121. (SCI Journal)

28. Forouhar, A. S., M. Liebling, A. Hickerson, A. Nasiraei-Moghaddam, H. J. Tsai, J. R. Hove, S. E. Fraser, M. E. Dickinson and M. Gharib*. 2006. The embryonic vertebrate heart tube is a dynamic suction pump. Science 312: 751-753 (SCI Journal)

29. Sabaawy, H. E., M. Azuma, L. J. Embree, H. J. Tsai, M. F. Starost and D. D. Hickstein*. 2006. TEL-AML1 transgenic zebrafish model of precursor B cell acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 103: 15166-15171. (SCI Journal)

30. Lin, C. Y., R. F. Yunag, H. C. Lee, W. T. Chen, Y. H. Chen and H. J. Tsai*. 2006. Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Dev. Biol. 299: 594-608. (SCI Journal)

31. Hu, C. Y., C. H. Yang, W. Y. Chen, C. J. Huang, H. Y. Huang, M. S. Chen and H. J. Tsai*. 2006. Egr1 gene knockdown affects embryonic ocular development in zebrafish. Mol. Vision 12: 1250-1258. (SCI Journal)

32. Chen, S. Y., C. Hsieh, S. Chu, C. Y. Lin, C. Ko, Y. Chen, H. J. Tsai, C. H. Hu and C. K. Sun*. 2006. Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo. J. Biomed. Opt. 11: 054022-8. (SCI Journal)

33. Chen, Y. H., Y. H. Wang, M. Y. Chang, C. Y. Lin, C. W. Weng, M. Westerfield and H. J. Tsai*. 2007. Multiple upstream modules regulate zebrafish myf5 expression. BMC Dev. Biol. 7: 1-14 (SCI Journal)

34. Ho, Y. L., Y. H. Shau, L. C. Lin, P. J. Huang, F. J. Hsieh and H. J. Tsai*. 2007. In vivo assessment of cardiac morphology and function in heart-specific green fluorescent zebrafish. J. Formos. Med. Assoc. 106: 181-185.

35. H. C. Lee, J. N. Tsai, P. Y. Liao, W. Y. Tsai, K. Y. Lin, C. C. Chuang, C. K. Sun, W. C. Chang, H. J. Tsai.*. 2007. Glycogen synthase kinase 3alpha and 3beta have distinct functions during cariogenesis of zebrafish embryo. BMC Dev Biol. 7:93.

36. N. G. Holtzman, J. J. Schoenebeck, H. J. Tsai and D. Yelon*. 2007. Endocardium is necessary for cardiomyocyte movement during heart tube assembly. Development.134, 2379-86. (SCI journal)

37. Y. L. Ho, Y. H. Lin, I. J. Tsai, F. J. Hsieh and H. J. Tsai*. 2007. In vivo assessment of cardiac morphology and function in heart-specific green fluorescent zebrafish. J. Formos Med. Assoc. 106, 181-186. (SCI journal)

38. H. J. Auman, H. Coleman, H. E. Riley, F. Olale, H. J. Tsai and D. Yelon*. 2007. Functional modulation of cardiac form through regionally confined cell shape changes. PLoS Biol. 5, e53. (SCI journal)

39. Y. H. Wang, C. K. Li, G. H. Le, H. J. Tsai* and Y. H. Chen*. 2008. Inactivation of the myogenic bHLH gene mrf4 in zebrafish leads to myofibril misalignment and motor axon growth disorganization in zebrafish embryos. Dev. Dynamics. 237, 1043-1050. (SCI journal)

40. Y. H. Chen*, and H. J. Tsai*. 2008. Myogenic regulatory factors Myf5 and Mrf4 of fish: Current status and perspective. J. Fish. Biol. 73, 1-19. (reviewed and invited article)(SCI journal)

41. S. L. Chen, S. S. Li, R. Huang and H. J. Tsai*. 2008. Conditional production of a functional fish hormone in the transgenic line of Nannochloropsis oculata. J. Phycol. 44: 768-776. (SCI journal)

42. S. S. Li, and H. J. Tsai*. 2008. Transgenic microalgae as a non-antibiotic bactericide producer to defense the bacterial pathogen infection in fish digestive tract. Fish Shellfish Immunol. (online) (SCI journal)

43. C. T. Tu, T. C. Yang and H. J. Tsai*. 2008. Nkx2.7 and Nkx2.5 function redundantly and are required for cardiac morphogenesis of zebrafish embryos. PLoS ONE (in print) (SCI journal)

44. H. Y. Huang, E. S. Dai, J. T. Liu, C. T. Tu, T. C. Yang and H. J. Tsai*. 2009. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene Dev. Dynamics 238: 232-240. (SCI journal)

45. C. Y. Fu, H. C. Lee and H. J. Tsai*. 2009. The molecular structures and expression patterns of zebrafish tropoin Ⅰ genes. Gene. Expr. Patterns (revised) (SCI journal)

46. C. Y. Lin, W. T. Chen, H. C. Lee, P. H. Yang, H. J. Yang and H. J. Tsai*. 2009. Transcription factor six1a plays function in craniofacial myogenesis of the zebrafish. Dev. Biol. (submitted) (SCI journal)