植物抗病基因座之定位與分析、分子標誌輔助育種

植物生長過程中經常受到各類病原菌的危害，種植抗病品種是經濟、有效且對環境友善的防治方式，也是發展安全農業不可或缺的一環。由於病原菌族群會不斷演化，因此必須深入瞭解植物抗病機制，持續找尋新穎抗性基因，並能掌握現有栽培品種所帶抗性，搭配對田間病原菌相的調查，才能正確使用抗病品種並適時進行輪替，讓抗性效果更持久。

• 水稻抗病基因座之定位與應用
  水稻為世界重要糧食作物，也是國內栽培面積最廣之作物。Magnaporthe oryzae引起的稻熱病可發生於水稻各個生育時期，在全球造成相當程度的經濟損失，也是臺灣一期稻作最主要的威脅；而Fusarium fujikuroi引起的徒長病，近年來則在臺灣及其他國家均有發生嚴重及抗藥性菌株之報導，在藥劑防治上存在隱憂。本研究室主要針對稻熱病和徒長病，利用數量遺傳分析策略，包括：連鎖定位 (linkage mapping)、全基因體關聯定位 (genome-wide association mapping, GWAS)，將植物體內複雜的抗感病反應，運用分子標誌或全基因體解序之方式，解構為個別的遺傳因子，深入瞭解其遺傳效應與機制。對於具潛力的抗性基因座，我們會進行細部定位、基因選殖及功能性驗證，以探索隱藏的候選基因或調控序列。除了學術上的探討，我們同時與農業試驗所、農業試驗所嘉義分所、高雄區農業改良場之育種專家們合作，將已知及新發現之抗性基因座資訊，以分子標誌輔助選拔之方式，實際運用於水稻的抗性改良。

• 草莓抗性機制及炭疽病菌致病機制之探討
  當前臺灣草莓栽培面臨之最大瓶頸為Colletotrichum spp.引起的炭疽病，可造成莖葉部黑斑、萎凋甚至苗期冠腐病。本研究室由抗感病品種草莓經水楊酸誘導之轉錄體資料，建構整體抗性網絡資料庫，並驗證具潛力之候選基因在炭疽病菌感染時是否也扮演重要角色。目前已透過將基因轉殖至阿拉伯芥過量表現之系統，初步證實草莓其中一個NPR-like gene對於防禦反應具負調控功能。我們目前也正著手瞭解炭疽病菌所分泌的毒素種類為何，是否為感染過程中的重要致病因子，期盼日後可應用於新穎防治策略之開發。

Localization and Analysis of Plant Disease Resistance Loci, Marker-Assisted Breeding

Plants are frequently threatened by various pathogens throughout their growth. Cultivating disease-resistant varieties is an economical, effective, and environmentally friendly control strategy essential for sustainable agriculture. Given the continuous evolution of pathogen populations, it is crucial to understand plant disease resistance mechanisms, discover novel resistance genes, and monitor the resistance in existing cultivars. This allows for the proper use of resistant varieties and timely rotation to maintain effective resistance.

• Localization and Application of Rice Disease Resistance Loci
  Rice, a globally significant staple crop, is extensively cultivated. Rice blast caused by Magnaporthe oryzae can affect rice at all growth stages, leading to substantial economic losses worldwide and is a major threat to Taiwan's first rice crop. Bakanae disease caused by Fusarium fujikuroi has also recently posed serious challenges due to reports of severe cases and resistant strains in Taiwan and other countries, raising concerns about chemical control. Our laboratory focuses on rice blast and bakanae disease, employing quantitative genetic analysis strategies such as linkage mapping and genome-wide association mapping (GWAS). These approaches decompose the complex host-pathogen interactions into individual genetic factors using molecular markers or whole-genome sequencing, enhancing our understanding of their genetic effects and mechanisms. We perform fine mapping, gene cloning, and functional validation for potential resistance loci to identify candidate genes or regulatory sequences. Collaborating with breeding experts from the Taiwan Agricultural Research Institute, Chiayi Agricultural Experiment Branch, and Kaohsiung District Agricultural Research and Extension Station, we apply known and newly discovered resistance loci in rice resistance improvement using marker-assisted selection.

• Strawberry Resistance Mechanisms and Anthracnose Pathogenic Mechanisms
  ​​​​​​​The main challenge in Taiwan's strawberry cultivation is anthracnose caused by Colletotrichum spp., leading to stem and leaf black spots, wilting, and crown rot in seedlings. Our laboratory constructs a comprehensive resistance network database using transcriptome data from salicylic acid-induced strawberry resistance and verifies whether candidate genes play significant roles during anthracnose infection. Preliminary results show that one NPR-like gene in strawberries negatively regulates defense responses when overexpressed in Arabidopsis. We are currently investigating the toxins secreted by anthracnose fungi to determine their role as key pathogenic factors during infection, with the aim of developing novel control strategies in the future.

樹木褐根病菌之生物學及防治管理

由褐根病菌Phellinus noxius引起的樹木褐根病，可危害超過200種以上的樹種，是目前林木、果樹、行道樹等的最大威脅之一。由於樹木根系受害後，容易因強風豪雨而倒伏，危害公共安全，但其地上部不一定呈現明顯病徵，因此造成早期偵測及防治管理上的困難。為釐清病害傳播模式，本研究室對褐根病菌進行全基因體定序組裝、簡單重複性序列 (simple sequence repeat) 分子標誌開發等，探討全臺灣菌株之遺傳多樣性，以分子證據釐清褐根病之可能傳播模式。我們正透過基因體及轉錄體之分析，瞭解褐根病菌之交配系統及致病機制。此外也結合植微系、植醫學程及樹藝社的熱心同學，給予實驗技術及實地觀察教育訓練，使其分工合作進行罹病地之病原菌含量檢測、生物防治、藥劑灌注效果評估、校園樹木健康檢查等工作，希望發展出環境友善之罹病地復育及整合性防治管理方法。

Biology and Management of Phellinus noxius

Tree brown root rot, caused by the fungus Phellinus noxius, affects over 200 tree species and poses a significant threat to forests, fruit trees, and street trees. The disease compromises tree root systems, leading to increased vulnerability to wind and rain, which can endanger public safety. However, the disease may not always present visible symptoms above ground, making early detection and management challenging.

To elucidate the disease transmission patterns, our laboratory conducts whole-genome sequencing and develops simple sequence repeat (SSR) molecular markers to explore the genetic diversity of Phellinus noxius strains across Taiwan. We analyze genomic and transcriptomic data to understand the mating systems and pathogenic mechanisms of the fungus. Additionally, we collaborate with enthusiastic students from plant microbiology, plant medicine programs, and the Tree Science Society, providing training in experimental techniques and field observations. This collaborative effort focuses on pathogen detection, biological control, chemical treatment evaluations, and campus tree health inspections, aiming to develop environmentally friendly and integrated management strategies for disease remediation.