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 期刊論文 (Publications)

Liao YY, Chuang YT, Lin HY, Lin NY, Hsu TW, Hsieh SC, Chen ST, Hung JS, Yang HJ, Liang JT, Huang MC*, Huang J*. GALNT2 promotes invasiveness of colorectal cancer cells partly through AXL. Molecular Oncology. 2022 Nov 21. doi: 10.1002/1878-0261.13347. Online ahead of print. (*Correspondence)

Li YN, Chen CW, Trinh-Minh T, Zhu H, Matei AE, Györfi AH, Kuwert F, Hubel P, Ding X, Manh CT, Xu X, Liebel C, Fedorchenko V, Liang R, Huang K, Pfannstiel J, Huang MC, Lin NY, Ramming A, Schett G, Distler JHW. Dynamic changes in O-GlcNAcylation regulate osteoclast differentiation and bone loss via nucleoporin 153. Bone Research. 2022 Jul 26;10(1):51. doi: 10.1038/s41413-022-00218-9.

Lin NY, Chen ST, Chang HL, Lu MY, Yang YL, Chou SW, Lin DT, Lin KH, Jou ST, Hsu WM, Huang MC, Chang HH*. C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma. Oncogenesis. 2022 Feb 15;11(1):8.

Kuo TC, Wu MH, Yang SH, Chen ST, Hsu TW, Jhuang JY, Liao YY, Tien YW*, Huang MC*. C1GALT1 high expression is associated with poor survival of patients with pancreatic ductal adenocarcinoma and promotes cell invasiveness through integrin αv. Oncogene. 2021 Feb;40(7):1242-1254. (*Correspondence)

Lin MC, Huang MC*, Lou PJ*. Anti-C1GALT1 Autoantibody Is a Novel Prognostic Biomarker for Patients With Head and Neck Cancer. Laryngoscope. 2021 Jan;131(1):E196-E202. (*Correspondence)

Lee PC, Chen ST, Kuo TC, Lin TC, Lin MC, Huang J, Hung JS, Hsu CL, Juan HF, Lee PH, Huang MC*. C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer. Oncogene. 2020 Mar;39(13):2724-2740. (*Correspondence)

Huang HC, Lin H, Huang MC*. Lactoferrin promotes hair growth in mice and increases dermal papilla cell proliferation through Erk/Akt and Wnt signaling pathways. Archives of Dermatological Research. 2019 Jul;311(5):411-420. (*Correspondence)

Hu WT, Yeh CC, Liu SY, Huang MC, Lai IR*. The O-glycosylating enzyme GALNT2 suppresses the malignancy of gastric adenocarcinoma by reducing EGFR activities. Am J Cancer Res. 2018 Sep 1;8(9):1739-1751.

Chen ST, Kuo TC, Liao YY, Lin MC, Tien YW*, Huang MC*. Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway. Oncogene. 2018 Nov;37(46):6041-6053. (*Correspondence)

Lin MC, Chien PH, Wu HY, Chen ST, Juan HF, Lou PJ*, Huang MC*. C1GALT1 predicts poor prognosis and is a potential therapeutic target in head and neck cancer. Oncogene. 2018 Oct;37(43):5780-5793. (*Correspondence)

Lin MC, Lin JJ, Hsu CL, Juan HF, Lou PJ*, Huang MC*. GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness. Oncogene. 2017 Jul 27;36(30):4243-4252.  (*Correspondence)

Lin TC^#, Chen ST^#, Huang MC, Huang J, Hsu CL, Juan HF, Lin HH, Chen CH^*. GALNT6 expression enhances aggressive phenotypes of ovarian cancer cells by regulating EGFR activity. Oncotarget. 2017 Jun 27;8(26):42588-42601.

Chou CH, Huang MJ, Liao YY, Chen CH*, Huang MC*. C1GALT1 seems to promote in vitro disease progression in ovarian cancer. International Journal of Gynecological Cancer. 2017 Jun;27(5):863-871. (*Correspondence)

Huang HC, Lin H, Huang MC*. The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells. International Journal of Molecular Medicine. 2017 Mar;39(3):595-602. (*Correspondence)

Ho WL, Hsu WM*, Huang MC*, Kadomatsu K, Nakagawara A. Protein glycosylation in cancers and its potential therapeutic applications in neuroblastoma. Journal of Hematology & Oncology. 2016 Sep 29;9(1):100. Review. (*Correspondence)

Liu SY, Shun CT, Hung KY, Juan HF, Hsu CL, Huang MC*, I-Rue Lai IR*. Mucin glycosylating enzyme GALNT2 suppresses malignancy in gastric adenocarcinoma by reducing MET phosphorylation. Oncotarget. 2016 Mar 8;7(10):11251-62. (*Correspondence)

Chen CH, Shyu MK, Wang SW, Chou CH, Huang MJ, Lin TC, Chen ST, Lin HH, Huang MC*. MUC20 promotes aggressive phenotypes of epithelial ovarian cancer cells via activation of the integrin β1 pathway. Gynecologic Oncology. 2016 Jan;140(1):131-7. (*Correspondence)

Chou CH, Huang MJ, Chen CH, Shyu MK, Huang J, Hung JS, Huang CS*, Huang MC*. Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway. Oncotarget. 2015 Mar 20;6(8):6123-35. (*Correspondence)

Huang MJ, Hu RH, Chou CH, Hsu CL, Liu YW, Huang J, Hung JS, Lai IR, Juan HF, Yu SL, Wu YM*,  Huang MC*. Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling. Oncotarget. 2015 Mar 20;6(8):5650-65. (*Correspondence)

Ho WL^#, Chou, CH^#, Jeng, YM, Lu MY, Yang YL, Jou ST, Lin DT, Chang HH*, Lin KH, Hsu WM*, Huang MC*. GALNT2 Suppresses Malignant Phenotypes Through IGF-1 Receptor and Predicts Favorable Prognosis in Neuroblastoma. Oncotarget. 2014 Dec 15;5(23):12247-59. (*Correspondence)

Liu CH, Hu RH, Huang MJ, Lai IR, Chen CH, Lai HS, Wu YM*, Huang MC*. C1GALT1 promotes invasive phenotypes of hepatocellular carcinoma cells by modulating integrin β1 glycosylation and activity. PLoS One. 2014 Aug 4;9(8):e94995. (*Correspondence)

Che MI#, Huang J#, Hung JS, Lin YC, Huang MJ, Lai HS, Hsu WM, Liang JT*. Huang MC*. β1,4-N-acetylgalactosaminyltransferase III modulates cancer stemness through EGFR signaling pathway in colon cancer cells. Oncotarget. 2014 Jun 15;5(11):3673-84. (*Correspondence)

Chen CH, Wang, SH, Liu CH, Wu YL, Wang WJ, Huang J, Hung JS, Lai IR, Liang JT, Huang MC*. β-1,4-galactosyltransferase III suppresses β1 integrin mediated invasive phenotypes and negatively correlates with metastasis in colorectal cancer. Carcinogenesis. 2014. Jun;35(6):1258-66. (*Correspondence)

Lu YC, Chen CN, Chu CY, Lu J, Wang BJ, Chen CH, Huang MC, Lin TH, Pan CC, Chen SS, Hsu WM, Liao YF, Wu PY, Hsia HY, Chang CC, Lee H. Calreticulin activates β1-integrin through fucosylation modification by fucosyltransferase-1 in J82 human bladder cancer cells. Biochem J. 2014 May 15;460(1):69-78.

Lin MC, Huang MJ, Liu CH, Yang TL*, Huang MC^*. GALNT2 enhances migration and invasion of oral squamous cell carcinoma by regulating EGFR glycosylation and activity. Oral Oncology. 2014 May;50(5):478-84. (*Correspondence)

Hung JS, Huang J, Lin YC, Huang MJ, Lee PH, Lai HS, Liang JT*, Huang MC*. C1GALT1 overexpression promotes the invasive behavior of colon cancer cells through modifying O-glycosylation of FGFR2. Oncotarget. 2014. Apr 30;5(8):2096-106. (*Correspondence)

Huang J, Che MI, Hung JS, Lin NY, Huang YT, Lin WC, Huang HC, Lee PH, Liang JT^*, Huang MC^*. The molecular chaperone Cosmc enhances malignant behaviors of colon cancer cells via activation of Akt and ERK. Molecular Carcinogenesis. 2014 Feb;53 Suppl 1:E62-71. (*Correspondence)

Ho WL, Che MI, Chou CH, Chang HH, Jeng YM, Hsu WM*, Lin KH*, Huang MC*. β1,3-N-Acetylglucosaminyltransferase-3 expression suppresses neuroblastoma cell migration and invasion and correlates with favorable outcomes of patients with neuroblastoma. Cancer Science. 2013 Dec;104(12):1600-8. (highlighted by MDLinx)(*Correspondence)

Liao WC, Wang YJ, Huang MC, Tseng GF. (2013) Methylcobalamin facilitates collateral sprouting of donor axons and innervation of recipient muscle in end-to-side neurorrhaphy in rats. PLoS ONE 8(9): e76302. doi:10.1371/journal.pone.0076302.

Wu YM#, Liu CH#, Huang MJ, Lai HS, Lee PH, Hu RH*, Huang MC*. C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization. Cancer Research. 2013 Sep 1;73(17):5580-90. *Correspondence (highlighted by SciBX: Science-Business eXchange; MDLinx)(*Correspondence)

Hsu WM, Huang CC, Wu PY, Lee H, Huang MC, Tai MH, Chuang JH. Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma. Cancer Letters. 2013 Aug 19;336(2):338-46.

Yeh MI, Huang HC, Liaw JH, Huang MC, Wu TH, Huang KF, Hsu FL. Ethosomes in hair dye products as carriers of the major compounds of black tea extracts. Int J Dermatol. 2013 Jul;52(7):868-75.

Chang HH#, Chen CH#, Chou CH, Liao YF, Huang MJ, Chen YH, Wang WJ, Huang J, Hung JS, Ho WL, Jeng YM, Che MI, Lee H, Lu MY, Yang YL, Jou ST, Lin DT, Lin KH, Hsu WM^*, Huang MC^* . β-1,4-galactosyltransferase III enhances invasive phenotypes via β1 integrin and predicts poor prognosis in neuroblastoma. Clinical Cancer Research.  2013 Apr; 19(7):1705-16. (*Correspondence)

Chen CH, Wang SW, Chen CW, Huang MJ, Hung JS, Huang HC, Lin HH, Chen RJ, Shyu MK*, Huang MC^*. MUC20 overexpression predicts poor prognosis and enhances EGF-induced malignant phenotypes via activation of the EGFR-STAT3 pathway in endometrial cancer. Gynecologic Oncology. 2013 Mar;128(3):560-7.(*Correspondence)

Lee JJ, Chen CH#, Chen YS#, Huang MJ, Huang J, Hung, JS, Chen MT, Huang MC*. COSMC is overexpressed in proliferating infantile hemangioma and enhances endothelial cell growth via VEGFR2. PLoS ONE 8(2): e56211. (2013. Feb. 12)(*Correspondence)

Yeh MI, Huang HC, Liaw JH, Huang MC, Huang KF, Hsu FL. Dermal delivery by niosomes of black tea extract as a sunscreen agent. Int J Dermatol. 2013 Feb;52(2):239-45.

Liao WC#, Chen CH#, Liu CH, Huang MJ, Chen CW, Hung JS, Chou CH, Chen CH, Che MI, Chang HM, Lan CT, Huang HC, Tseng GF, Shyu MK^*, Huang MC^*. Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion. Placenta. 2012 Dec;33(12):1005-11. (*Correspondence)

Shih YY, Nakagawara A, Lee H, Juan HF, Jeng YM, Lin DT, Yang YL, Tsay YG, Huang MC, Pan CY, Hsu WM, Liao YF. Calreticulin Mediates Nerve Growth Factor-Induced Neuronal Differentiation. Journal of Molecular Neuroscience. 2012 Jul;47(3):571-81.

Wu YM, Liu CH, Hu RH, Huang MJ, Lee JJ, Chen CH, Huang J, Lai HS, Lee PH, Hsu WM, Huang HC, Huang MC^*. Mucin glycosylating enzyme GALNT2 regulates the malignant character of hepatocellular carcinoma by modifying the EGF receptor. Cancer Research. 2011 Dec 1;71(23):7270-9. (*Correspondence)

Hsu WM#, Che MI#, Liao YF, Chang SH, Chen CH, HuangYM, Jeng YM, Huang J, Quon, MJ, Lee H, Huang HC, Huang MC*. B4GALNT3 expression predicts a favorable prognosis and suppresses cell migration and invasion via β1-integrin signaling in neuroblastoma. American Journal of Pathology. 2011 Sep;179(3):1394-404.(*Correspondence)

Shyu MK, Chen CW, Lin NY, Liao WC, Chen CH, Lin CJ, Huang HC, Lee JJ, Huang MJ, Tseng GF, Shih JC, Lee CN, Hsieh FJ, Huang MC*. MUC1 expression is elevated in severe preeclamptic placentas and suppresses trophoblast cell invasion via β1-integrin signaling. Journal of Clinical Endocrinology and Metabolism. 2011 Dec 1;96(12):3759-67. (*Correspondence)

Chen ST, Jeng YM, Chang CC, Chang, HH, Huang MC, Juan, HF, Hsu, CH, Lee H, Liao YF, Lee YL, Hsu WM, Lai HS. Insulin-Like Growth Factor II mRNA-Binding Protein 3 expression predicts unfavorable prognosis in patients with neuroblastoma. Cancer Science. 2011 Dec;102(12):2191-8.

Chang TT#, Shyu MK#, Huang MC#, Hsu CC, Yeh SY, Chen MR, Lin CJ. Hypoxia-mediated downregulation of OCTN2 and PPARα expression in human placentas and in BeWo cells. Molecular Pharmaceutics. 2011 Feb 7;8(1):117-25. (# Co-first authors)

Chang HH, Lee H, Hu MK, Tsao PN, Juan HF, Huang MC, Shih YY, Wang, BJ, Jeng YM, Chang C, Huang SF, Tsay YG, Hsieh FJ, Lin KH, Hsu WM. Notch1 expression predicts an unfavorable prognosis and serves as a therapeutic target of patients with neuroblastoma. Clinical Cancer Research. 2010 Sep 1;16(17):4411-20.

Huang J, Che MI, Huang YT, Shyu MK, Huang YM, Wu YM, Lin WC, Huang PH, Liang JT, Lee PH, Huang MC*. Overexpression of MUC15 Activates Extracellular Signal–Regulated Kinase 1/2 and Promotes the Oncogenic Potential of Human Colon Cancer Cells. Carcinogenesis. 2009 Aug;30(8):1452-8. (*Correspondence)

Shyu MK, Lin MC, Liu CH, Fu YR, Shih JC, Lee CN, Chen HY, Huang J, Huang MC*, Hsieh FJ. MUC1 expression is increased during human placental development and suppresses trophoblast-like cell invasion in vitro. Biology of Reproduction. 2008 Aug;79(2):233-9. (*Correspondence) ("Highlights" article)

Shyu MK, Lin MC, Shih JC, Lee CN, Huang J, Liao CH, Huang IF, Chen HY, Huang MC*, Hsieh FJ. Mucin 15 is expressed in human placenta and suppresses invasion of trophoblast-like cells in vitro. Human Reproduction. 2007 Oct;22(10):2723-32. (*Correspondence)

Huang HC, Chen HY, Chen CY, Shen TL, Huang J, Lin NY, Sum SY, Che MI, Huang MC*. Ammonium hydroxide extracts from black tea inhibit growth, migration, and invasion of colon cancer cells. Journal of Food Biochemistry. 2008 Apr;32:201-15. (*Correspondence)

Huang J, Liang JT, Huang HC, Shen TL, Chen HY, Lin NY, Che MI, Lin WC, Huang MC^*. β1,4-N-acetylgalactosaminyltransferase III enhances malignant phenotypes of colon cancer cells. Molecular Cancer Research. 2007 Jun;5(6):543-52. (*Correspondence)

Huang MC^*, Chen HY, Huang HC, Huang J, Liang JT, Shen TL, Lin NY, Ho CC, Cho IM, Hsu SM. C2GnT-M is downregulated in colorectal cancer and its re-expression causes growth inhibition of colon cancer cells. Oncogene. 2006 Jun 1;25(23):3267-76. (*Correspondence)

  專利與技轉 (Patents and Technology Transfer)

  實驗室技術 (Techniques)

細胞生物：Cell culture, gene transfection (calcium phosphate, DEAE dextran, liposome, adenovirus, electroporation), hair follicle organ culture, immunocytochemistry, paraffin-embedding, cryosection, H&E staining, immunohistochemistry (IHC), in situ hybridization, flow cytometry, migration assay, soft agar assay, invasion assay, MTT/WST-1 assay, cell cycle analysis, apoptosis assays, cell adhesion assay, rolling assay, fluorescence microscopy, confocal microscopy

分子生物： RNA and DNA extraction, RT-PCR, real-time PCR, methylation-specific PCR, gene cloning, transformation, electroporation, library construction and screening, Northern blot, Southern blot, Western blot, Northwestern blot, surface biotinylation/immunoprecipitation, DNA sequencing, siRNA knockdown, inducible expression system, expression cloning, CsCl banding, yeast two-hybrid system, phage display, site-directed mutagenesis, radio-isotope handling

生物化學：protein expression in E. coli, yeast, mammalian cells, purification, HPLC, FPLC, protein ID identification, enzyme activity assay, carbohydrate purification, glycosylation analysis, lipid and glycolipid purification, TLC, lipid raft purification, SDS-PAGE, 2D, MMP zymography

動物實驗： in vivo tumor xenograft, in vivo metastasis assays (IV and intrasplenic injection), transgenic mice, wound healing models, hair growth models

免疫實驗：ELISA, mAb generation, polyclonal antibody generation (mouse, rabbit), PBMC/neutrophil/bone marrow isolation, skin inflammation, acute inflammation model