錢宗良老師辦公室
Chung-Liang Chien's Home Page
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2000-2010研究主題 2011起- 研究主題 |
| 1. 胚胎幹細胞生物學 (Biology of Embryonic Stem (ES) cells) |
(A)  1.Nestin-positive neural progenitors and Tuj1-positive neurons differentiated from mouse ESCs. 2.RA-Fgf/Erk signaling in early neural differentiation. (Impaired Neural Differentiation Potency by Retinoic Acid Receptor-α Pathway Defect in Induced Pluripotent Stem Cells. Cell Reprogram.2014.) (B)   1.LHX2 was co-localized with PAX6 in differentiating hESCs in differentiation hESCs. 2.Model for LHX2-mediated neural differentiation in hESCs. (LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. Nucleic Acids Research. 2013) |
| 2.癌症細胞生物學 (Cancer Cell Biology) |
 1.Expression of GFAP detected in C6 cells after IBMX induction. 2.The possible mechanism by which miRNA-30c regulates GFAP expression. (The role of microRNA-30c in the self-renewal and differentiation of C6 glioma cells.Stem Cell Research. 2015.) |
| 3. 神經退化之細胞及動物模式 (Cellular and Animal Models for Neuronal Degeneration) |
(A)  Evaluation of hematoma-associated injury area and brain tissue loss after cell implantation by MRI. (A) MRI and corresponding brain section at one day after ICH. The abnormal signal corresponds to the hematoma injury area is marked. (B) The 3T3-BDNF-EGFP treated group has less brain tissue loss. (Brain-derived Neurotrophic Factor-Transfected and Nontransfected 3T3 Fibroblasts Enhance Migratory Neuroblasts and Functional Restoration in Mice with Intracerebral Hemorrhage. J. Neuropathol. Exp. Neurol. 2012) (B)   1.Establish an EPO-overexpressing NIH/3T3 fibroblast cell line. 2.Rearrangement and disaggregation of neurofilaments, such as NF-M and α-INT-EGFP, were found in the group supplied with EPO-3T3-EGFP conditioned medium. (Erythropoietin produced by genetic-modified NIH/3T3 fibroblasts enhances the survival of degenerating neurons. Brain and Behavior, 2015) Video 1: Time-lapse_ vehicle of PC12-INT-EGFP cells (Control) Video 2: Time-lapse_ PC12-INT-EGFP cells with EPO-3T3-EGFP conditioned media treatment |
| 4. 神經發育之動物模式 (Animal Models of Neural Development) |
(A) 
 1.chkINA is the main neuronal intermediate filament in the process of cerebellar granule neurons. 2.Neuronal Intermediate Filaments Proteins in the Developing Chicken Cerebellum. (Molecular Cloning and Characterization of Chicken Neuronal Intermediate Filament Protein α-Internexin. J. Comp. Neurol. 2013) (B)  The distribution of zebrafish gefiltin at 5 dpf by whole-mount IHC. (The Developmental Distribution of Neuronal Intermediate Filament inaa and inab in the Zebrafish Retina. J Comp Neurol. 2016 ) |