Resources in Hu lab

Thesis writing and some related information

• I have written a guideline for writing thesis (or any manuscript): [pdf format file]
  
• Here is a list of terms with Chinese translations that are commonly used in evolutionary and phylogenetic studies: [pdf format file]
  
• Here is the angiosperm family names with Chinese translations arranged by APG (APG 1998; Bremer 2001): [pdf format file]
  
• Here is a little tip for using Endnote
  

Plant reproductive biology

• Here is a reference collection for the studies of plant reproductive biology in Taiwan.

Floral evo-devo

• Some list of MADS-box genes, etc.

Protocols

This is the collection of protocols used in Hu lab. The source of the original protocols are marked.

• Anatomy/morphology related (in pdf format)
  • Sectioning
  • Staining by Tol-Blue
  • Staining by PAS (Periodic acid / Schiff's) reaction
  • Embedding samples in parafilm
  • SEM sample preparation

• Molecular biology related (in pdf format)
  • DNA isolation from plants (Standard CTAB protocol)
  • DNA isolation from rainforest plants (modified from Scott & Playford 1996)
  • RNA isolation from plants (LiCl method)
  • RNA isolation from plants (TriZOL and PineTree methods - by Shu-Hsing Wu's lab)
  • RNA isolation (Concert plant RNA reagent, Invitrogen manual)
  • RNA gel making
  • Competent cell making (From Erh-Min Lai's lab)
  • Heat shock transformation
  • Quick screening (bacterial clones)
  • Purifying plamids (miniprep)
  • Northern blot
  • In situ protocols
  • Common solution preparation

[EMAIL: jmhu@ntu.edu.tw]