Nanowire Transistor-based Ultrasensitive Virus Detection with Reversible Surface Functionalization.
The surface of a 3-mercaptopropyltrimethoxysilane (MPTMS)/SiNW-FET was modified with mAbH5 to become mAbH5/SiNW-FET through a disulfide linker. After the AIV detection, the AIV-mAbH5 complex on the SiNW-FET was removed by dithiothreitol (DTT) washing to return the device to the MPTMS/SiNW-FET.
Chem. Asian J. 2012, in press.
Rapid and Specific Influenza Virus Detection by Functionalized Magnetic Nanoparticles and Mass Spectrometry
The timely and accurate diagnosis of specific influenza virus strains is crucial to effective prophylaxis, vaccine preparation and early antiviral therapy. In conjugation with the immunoassay utilizing monoclonal antibody, mass spectrometry is an alternative to identify proteins derived from a target influenza virus. Taking advantage of the large surface area-to-volume ratio, antibody-conjugated magnetic nanoparticles can act as an effective probe to extract influenza virus for sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and on-bead mass spectrometric analysis. J. Nanobiotech. 2011, 9, 52.
Synthesis and
Evaluation of a New Fluorescent Transglycosylase Substrate: Lipid
II-based Molecule Possessing a Dansyl-C20 Polyprenyl Moiety
A novel fluorescent lipid
II-based substrate for transglycosylases (TGases) is designed, via
an imaginary transform A, to incorporate a dansyl group to mimic the
diprenyl moiety in the lipid chain. This fluorescent substrate is
readily utilized in the functional assay of TGases without an
additional treatment with another enzyme of N-acetylmurmidase.
Org. Lett. 2010, 12, 1608–1611.
A
Fluorescence Sensor for Detection of Geranyl Pyrophosphate by
Chemo-Ensemble Method
A chemo-ensemble
system using FRET as the sensing mechanism to detect isoprenoid
pyrophosphates. The hexaamide receptor molecule has a well-defined
structure with pseudo-tetrahedral cleft to accommodate the
pyrophosphate, and incorporates two long hydrocarbon chains to
enhance the interactions with the aliphatic moiety of GPP. The
coumarin phosphate is selected as an appropriate fluorescence
indicator because it binds modestly with the receptor to render a
FRET from the pyrene rings. GPP selectively replaces coumarin
phosphate, but other anions including CH3CO2–,
C10H21CO2–, C11H23CO2–,
F–, Cl–, Br–, SCN–, NO3–,
and ClO4– cannot. This chemo-ensemble system
provides an efficient, selective and sensitive method for detection
of GPP. J. Org. Chem. 2009, 74, 895–898.
Phospholipid-Induced
Aggregation and Anthracene Excimer Formation
The zinc complex of anthryl bis(dipicolylamine) ( 1 ) aggregates upon binding with long-chain aliphatic phosphates and displays anthracene excimer fluorescence, which provides a new strategy toward detection of the biologically important lysophosphatidic acid in aqueous solution. Org. Lett. 2008, 10, 4401-4404.
Production of Antibodies for Selective Detection of Malachite Green and the Related Triphenylmethane Dyes in Fish and Fishpond Water
This study provides a practical method for production of the antibodies against malachite green (MG) and its primary metabolite leucomalachite green (LMG). Two ELISA kits are constructed with the MG and LMG antibodies for detection of the residual MG and LMG in fish muscle and fishpond water. The detection limit is established at the level of 0.05 μg/L for both MG and LMG. Our ELISA kits show the advantages of good specificity, high sensitivity, and convenience in rapid screening of MG and LMG residues. The sample of fishpond water, without extraction or prior preparation, is directly assayed by the ELISA kit. More then 80 fish samples can be simultaneously tested in a kit. The toxic crystal violet and its metabolite leucocrystal violet of illegal use in aquaculture are detected by our prepared MG and LMG antibodies, whereas the antibodies do not cross-react with common antibiotics, sulfonamides, and benzene derivatives.
J. Agric. Food Chem.2007, 55, 8851.
Fluorescent and Circular Dichroic Detection of Monosaccharides by Molecular Sensors: Bis[(Pyrrolyl)ethynyl]naphthyridine and Bis[(Indolyl)ethynyl]naphthyridine
The push - pull conjugated molecules BPN and BIN adopting daad relays of proton donors(d) and acceptors(a) form multiple hydrogen-bonding complexes with various monosaccharides that possess complementary adda sequences. On the other hand, the CD-silent BIN molecule binds with monosaccharides to form the CD-active multiple hydrogen- bonding complexes, which exhibit the remarkable chirality dependent helicities consistent with the prediction by the ab initio approaches. J. AM. CHEM. SOC. 2004, 126, 3559–3566.
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