Chung-Liang Chien's HomePage
Department of Anatomy and Cell Biology, College of Medicine,
National
Taiwan University
Contents(top)
Current Projects and Publications
Molecular Biology course:
Transgenic techniques 2002
1. Transgenic mice
2. ES Knockout
3. ES positive-negative selection
4. Conditional knockout and knockin
Cell Biology Course: Mammalian
Development 2002
Biotechnique Course 2002: Genetic Manipulation in Mouse (II)
Homepage for The Core Laboratory of Confocal Microscope
Associate Professor
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University,
1, Section 1, Jen-Ai Road, Taipei, Taiwan 100
Key responsibilities
1. Principle investigator of the molecular biology
laboratory (Room 636)
and Core laboratory of Confocal Microscope
(Room 1410)
2. Anatomy Course coordinator for Departments of Medical Technology, Nursing, Public Health, Pharmacy, Occupation Therapy, and Physical Therapy.
Courses
Anatomy, Histology, Cell Biology, Molecular Biology, Biotechniques, Cell and Tissue Ultrastructure, and Introduction of Cell Biology.
Cell
Biology (Cytoskeletons, intermediate filaments, microtubule-associated
proteins)
Cultured cerebellar granule neurons. Spider-web like neuronal processes were labeled with antibody to neuronal intermediate filament protein, internexin.
Developmental
Neuroscience (Transgenic approach)
A Chimeric Mouse carrying the targeted internexin gene.
Immunostaining of transgene product (rat internexin) in the transgenic mouse cerebellum. Arrows indicate the swollen axons (torpedo) from Purkinje cells.
· Cancer Biology
Subcellular
localization of oncoproteins in the tumor cells
Retinoid induced the
apoptosis of tumor cells
E-mail address: clc@ha.mc.ntu.edu.tw
Web address: http://med.mc.ntu.edu.tw/~anatomy/chien/chien.html
Office Phone: +886-2-2312-3456 Ext. 8193
+886-2-2394-8679
Office Fax: +886-2-2391-5292
Current Projects and Publications
1. Immunoelectron microscopy and in situ hybridization studies of neuronal intermediate filaments in developing mouse cerebella (NSC-86-2314-B-002-152)
2. The expression and distribution of neuronal intermediate filament proteins in the developing mouse central nervous system (NSC-87-2314-B-002-244)
3. Histology Atlas: CD-ROM and on-line education (NSC-87-2511-S-002-007)
4. A transgenic animal model for cerebellar neuropathology (NSC-88-2314-B-002-119)
5. Effects of retinoic acid on the apoptosis of human bladder carcinoma T24 cells (NSC-89-2314-B-002-130)
6.. Transgenic mice of Microtuble associated proteins (Collaborated with Dr. Hirokawa at University of Tokyo)
7.
Functional studies of Microtubule Associated protein 1A (NSC-89-2320-B-002-265)
Publications:
1. Chien, C.L., Y.P. Chau, and K.S. Lu. 1991.
Ultrastructural studies on the barrier properties of the paraganglia in the rat
recurrent laryngeal nerve. Acta Anat. 141:262-268. (SCI)
2. Chau, Y.P., C.L. Chien, and K.S. Lu. 1991.
The permeability of capillaries among the small granule-containing cell in rat
superior cervical ganglia: an ultrastructural lanthanum tracer study. Histol.
Histopathol. 6:261-268. (SCI)
3. Lu, K.S., C.L. Chien, and Y.P. Chau. 1992.
Effects of 5-hydroxydopamine and 6-hydroxydopamine on the ultrastructure of
Type I cells in paraganglia of the rat recurrent laryngeal nerve. Arch. Histol.
Cytol. 55:57-65. (SCI)
4. Chien, C.L. and R.K.H. Liem. 1994.
Characterization of the mouse gene encoding the neuronal intermediate
filament protein, alpha-internexin. Gene 149:289-292. (SCI)
5. Chien, C.L. and R.K.H. Liem. 1995. The
neuronal intermediate filament, alpha-internexin is transiently expressed in
amacrine cells in the developing mouse retina. Exp. Eye Res. 61:749-756. (SCI)
6. Chien, C.L., C.A. Mason, and R.K.H. Liem.
1996. alpha-internexin is the only neuronal intermediate filament expressed in developing
cerebellar granule neurons. J. Neurobiol. 29:304-318. (SCI)
7. Wang, S.M., T.H Fong, S.Y. Hsu,
C.L. Chien and J.C. Wu. 1997. Reorganization of a novel vimentin-associated
protein in 3T3-L1 cells during adipose conversion. J. Cellular Biochem. 67:84-91.
(SCI)
8. Wei, H.J., T.H. Su, C.L. Chien, and C.R.
Tzeng. 1997. Florescence in situ hybridization (FISH) as a method to detect
aneuploid cells. Fetal Diagn. Ther. 12:309-313. (SCI)
9. Pekny M., C. Eliasson, C.L. Chien, L.G.
Kindblom, R. Liem, A. Hamberger, and C. Betsholtz. 1998. GFAP-deficient
astrocytes are capable of stellation in vitro when cocultured with neurons and
exhibit a reduced amount of intermediate filaments and an increased cell
saturation density. Exp. Cell Res. 239:332-343. (SCI)
10. Ho, C.L., C.L. Chien, K. Brown, and R.K.H.
Liem. 1998. Studies of carboxyl-terminal truncation mutants of the neuronal
intermediate filament protein, peripherin. J. Neurochem. 70:1916-1924. (SCI)
11. Chien, C.L., T.H. Lee, and K.S. Lu. 1998.
The distribution of neuronal intermediate filament proteins in the developing
mouse olfactory system. I. Neurosci. Res. 54:353-363. (SCI, NSC
87-2314-B-002-244)
12. Ching, G.Y., C.L. Chien, R. Flores, and
R.K.H. Liem. 1999. Overexpression of alpha-internexin causes abnormal
neurofilamentous accumulations and motor coordination deficits in transgenic
mice. J. Neurosci. 19:2974-2986. (SCI, NSC 88-2314-B-002-119)
13. Shih S.C., C.L. Chien, K.W. Tseng, S.C.
Lin, and C.R. Kao. 1999. Immunohistochemical studies of transforming growth
factor-? and its receptors in the gastric mucosa of patients with refractory
gastric ulcer. J. Formosan Medical Assoc. 98:613-620.
14. Yang, M.F., C.L. Chien, and K.S. Lu. 1999.
Morphological, immunohistochemical and quantitative studies of murine brain
mast cells after mating. Brain Res. 846:30-39.
· 15.
Chou H.C., C.L. Chien, H.L. Huang, and K.S. Lu. 2001.Effects of zinc deficiency
on the vallate papillae and taste buds in rats. J Formos Med Assoc.
100:326-335.
16. Chou H.C., C.L. Chien, and K.S. Lu. 2001.
The distribution of PGP9.5, BDNF and NGF in the vallate papilla of adult and
developing mice. Anat. Embryol. 204:161-169.
17. Chien, C.L., P. Wang, H.S. Yin, J.H. Lue,
J.C. Wu, S.T. Hsieh, and K.S. Lu. 2001. Evaluation of anatomy course from the
students of allied health sciences. J. Med. Education 5:124-134.
Conference Abstracts:
1. Chien, C.L. and R.K.H. Liem. 1993.
Sequence, structure and targeted mutagenesis of the mouse neuronal
intermediate filament alpha-internexin gene. Mol. Biol. Cell 4
(Supplement):175a.
2. Chien, C.L. and R.K.H. Liem. 1994.
Targeted mutagenesis of neuronal intermediate filament genes by homologous
recombination. Mol. Biol. Cell 5 (Supplement):49a. (SCI)
3. Chien, C.L., T.H. Lee, and K.S. Lu. 1997.
The distribution of neuronal intermediate filament proteins in the developing
mouse olfactory bulb. Mol. Biol. Cell 8 (Supplement):279a. (SCI, NSC
86-2314-B-002-152)
4. Ching, G.Y., C.L. Chien, R.L. Flores, and
R.K.H. Liem. 1997. Abnormalities in brain and behavior of transgenic mice
overexpressing neuronal intermediate filament protein alpha-internexin. Mol.
Biol. Cell 8 (Supplement):278a.
5. Ching, G.Y., C.L. Chien, B. Rosen, and
R.K.H. Liem. 1998. Neuronal abnormalities in transgenic mice expressing alpha-internexin
/ beta- galactosidase fusion protein. Mol. Biol. Cell 9 (Supplement):159a.
6. Chien, C.L., K.S. Lu, T.W. Chen, and
Yi-Shan Lin. 2000. Intermediate filament is the first cytoskeleton affected by
caspase in the early apoptotic process of human bladder carcinoma T24 cells.
Mol. Biol. Cell 11 (Supplement):535a.
1999-1999 University of Tokyo. Research Scientist Cell Biology
1992-1995 Columbia University. Ph.D. Pathology
1990-1992 Columbia University. M.Ph. Pathology
1989-1990 Columbia University. M.A. Pathology
1986-1989 National Taiwan University. M.S. Anatomy
1980-1984 National Taiwan University. B.S. Zoology
Classical Music, Chess, Computer
Last Revised: 5-25-2002