近年研究主題

  1. 次級細胞壁生長中的轉錄調節
  2. 木質部發育、木材生成
  3. 生物能源

研究室簡介

木材是紙漿製造、建材、生質能源最重要的原料。木材形成是一個複雜的發育過程,其中包含有次級木質部細胞從維管束形成層分化、以及次級細胞壁增厚。多細胞生物的生長和發育過程是經由多層級調控因子所調控,研究木材發育的多層級調控將可獲得更準確的知識進而對木材的特性改造。透過使用毛果楊木質部的原生質體,我們將研究木材形成和次級細胞壁增厚的遺傳調控網絡。

在開花木本植物中,木質部由三種細胞組成:纖維細胞,導管細胞,放射細胞。這三種細胞是從維管束形成層分化而成,並且有不同的功能。纖維細胞提供支撐,導管細胞運輸水分,放射細胞儲存營養。在這三種細胞中,纖維細胞和導管細胞為主要多數,特別是纖維細胞佔木質部總細胞的60-80%。我們將會只用雷射顯微切割技術進行細胞的分離及蒐集,進而研究木質部發育的機制。

木材是世界上含量最多的生物量的物質並且可以再生,透過木材形成酒精將可提供可觀的生物能源,所以透過了解木材形成,木質部發育,我們將可以更準確的改良木材特性以提供生物能源製造效率和產量。

Research Interests

  1. Transcriptional regulation in secondary cell wall
  2. Xylem development, Wood formation
  3. Biofuel

Laboratory

Wood is the most important raw material for the production of pulp for paper making, of lumber for construction and furniture, and of ethanol for energy. Wood formation is a complex developmental process involving differentiation of secondary xylem cells from the vascular cambium, followed by thickening of the cell wall (secondary cell wall). Growth and development in multicellular organisms are regulated at many levels by transacting molecules following well-structured regulatory hierarchies. Understanding the regulatory hierarchy of wood formation will offer novel and more precise genetic approaches to improve the productivity of forest trees. Using Populus trichocarpa protoplasts from stem-differentiating xylem (SDX) as a model, we have begun to reveal the transcriptional regulatory network involved in secondary cell wall formation.

Wood is secondary xylem and, in angiosperm trees, consists of three types of cells, fiber, vessel and ray. In vascular cambium, a thin layer of undifferentiated cells, the fusiform initials or the stem cells, divides and differentiates to give rise to all the three wood cell types. The three types of cells have unique functions. Fiber cells provide support, vessels transport water, and ray cells store nutrients and extractives. Of the three cell types, fibers and vessel elements are the two major ones, with fibers accounting for approximately 60 to 80% of the wood volume. We will use Laser Capture Microdissection (LCM) to isolate vascular cambium, fiber, vessel, and ray cells to study the mechanism of xylem development.

Biofuels and biomass depend on plants as feedstocks for fermentation and other energy related processing. Wood, the most abundant form of plant biomass, can be produced sustainably on land that is not suitable for food crops. Understanding wood formation would improve plant productivity, production of materials, energy, and food. A sustainable and abundant wood supply continues to be an essential national resource.




代表著作

Publications

  1. Li S.*, Lin Y.C.J.*, Wang P., Zhang B., Li M., Chen S., Shi R., Tunlaya-Anukit S., Liu X., Wang Z., Dai X., Yu J., Zhou C., Liu B., Wang J.P., Chiang V.L., Li W. (2018) Histone Acetylation Cooperating with AREB1 Transcription Factor Regulates Drought Response and Tolerance in Populus trichocarpa. The Plant Cell DOI: https://doi.org/10.1105/tpc.18.00437. (* Co-first author) (IF=9.378; R/C=6/223, PLANT SCIENCES).
  2. Yan X., Liu J., Kim H., Liu B., Huang X., Yang Z., Lin Y.C.J., Chen H., Yang C., Wang JP., Muddiman D.C., Ralph J., Sederoff R.R., Li Q., Chiang V.L. (2018) CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa. New Phytologist doi: 10.1111/nph.15505 (IF=7.433; R/C=7/223, PLANT SCIENCES)
  3. Wang J.P., Matthews M.L., Williams C.M., Shi R., Yang C., Tunlaya-Anukit S., Chen H.C., Li Q., Liu J., Lin C.Y., Naik P., Sun Y.H., Loziuk P.L., Yeh T.F., Kim H., Gjersing E., Shollenberger T., Shuford C.M., Song J., Miller Z., Huang Y.Y., Edmunds C.W., Liu B., Sun Y., Lin Y.C.J., Li W., Chen H., Peszlen I., Ducoste J.J., Ralph J., Chang H.M., Muddiman D.C., Davis M.F., Smith C., Isik F., Sederoff R., Chiang V.L. (2018) Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis. Nature Communications 9, 1579 (IF=12.1; R/C=3/64, MULTIDISCIPLINARY SCIENCES).
  4. Lin Y.C.J.*, Chen H.*, Li Q.*, Shi R., Tunlaya-Anukit S., Shuai P., Li W., Li H., Sun Y.H., Sederoff R.R., Chiang V.L. (2017). Reciprocal cross-regulation of VND and SND multi-gene TF families in wood formation in Populus trichocarpa. PNAS 114(45):E9722-E9729 (* Co-first author) (IF=9.7; R/C=4/64, MULTIDISCIPLINARY SCIENCES).
  5. Shi R., Wang J.P., Lin Y.C., Li Q., Sun Y.H., Chen H., Sederoff R.R., Chiang V.L. (2017). Tissue and cell-type co-expression networks of transcription factors and wood component genes in Populus trichocarpa. Planta 245, 927–938 (IF=3.2; R/C=28/197, PLANT SCIENCES).
  6. Wang P.J.*, Chuang L.*, Loziukc P.L., Chen H., Lin Y.C., Shi R., Qu G.Z., Muddiman D.C., Sederoff R.R., Chiang V.L. (2015). Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis. PNAS, 112, 8481–8486. (* Co-first author). (IF=9.4; R/C=4/57, MULTIDISCIPLINARY SCIENCES).
  7. Lin, Y.C., Li, W., Li, Q., Chen, H., Sederoff, R.R., and Chiang, V.L. (2014). A simple improved-throughput xylem protoplast system for studying wood formation. Nature Protocols, 9, 2194-2205. (IF= 9.6; R/C=2/79, BIOCHEMICAL RESEARCH METHODS).
  8. Li, W., Lin, Y.C., Li, Q.Z., Chen, H., Sederoff, R.R., and Chiang, V.L. (2014). A robust chromatin immunoprecipitation protocol for studying transcription factor–DNA interactions and histone modifications in wood-forming tissue. Nature Protocols, 9, 2180-2193. (IF= 9.6; R/C=2/79, BIOCHEMICAL RESEARCH METHODS).
  9. Chen, H.-C.*, Song, J.*, Wang, P.J.*, Lin, Y.C., Ducoste, J., Shuford, M.C., Liu, J., Li Q., Shi, R., Nepomuceno, A., Isil, F., Muddiman, C.D., Williams, C., Sederoff, R.R., and Chiang, V.L. (2014). Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric acid: CoA Ligase (4CL) Protein Complex Formation, Regulation and Numerical Modeling. The Plant Cell. 26, 876-893. (* Co-first author) (IF=9.3; R/C=4/204, PLANT SCIENCES).
  10. Lin, Y.C.*, Li, W.*, Sun, Y.H., Kumari, S., Wei, H.R., Li, Q.Z., Tunlaya-Anukit, S., Sederoff, R.R., and Chiang, V.L. (2013). SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. The Plant Cell 25, 4324-4341. (* Co-first author). (IF=9.6; R/C=4/204, PLANT SCIENCES).
  11. Li Q.*, Lin Y.C.*, Sun Y. H., Song J., Chen H., Zhang X. H., Sederoff R. R., and Chiang V. L. (2012). Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. PNAS 109, 14699-14704. (* Co-first author) (IF=9.7; R/C=4/57, MULTIDISCIPLINARY SCIENCES).
  12. Wang J.P., Shuford C.M., Li Q., Song J., Lin Y.C., Sun Y.H., Chen H.C., Williams C.M., Muddiman D.C., Sederoff R.R., Chiang V.L. (2012). Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC-MS/MS based protein quantification and metabolic flux analysis. Planta 236(3):795-808. (IF=3.3; R/C=28/197, PLANT SCIENCES).

開設課程

  • 普通生物學
  • 植物生理學

實驗室留影


2017實驗室慶祝中秋節


2018 畢業典禮

Photos


2017~Celebration for moon festival


2018 Graduation ceremony