近年研究主題

  1. 中腦正腎上腺素神經元的放電特徵與其離子、分子機制研究,以及胜肽類神經傳導物質對它們的調控。
  2. 中樞神經系統突觸傳導與可塑性機制研究。

研究室簡介

我們實驗室主要利用電生理學(whole-cell patch clamp recording)的方法與腦組織薄片並配合其他現代生物學技術來研究中腦正腎上腺素神經元的放電特徵與其離子、分子機制,以及胜肽類神經傳導物質對它們的調控。中腦正腎上腺素神經元群,包括藍斑核(A6)與A7細胞群是中樞神經系統中主要的正腎上腺素的來源。正腎上腺素系統對諸如睡眠/清醒狀態、學習記憶、痛覺與其他感覺訊息的傳遞…等等大腦功能,扮演重要的調節角色。因此對這群神經元的興奮性如何被調控與相關機制,將有助於我們對大腦這些功能運作的了解。我們另一個研究主題是:中樞神經系統突觸傳導與可塑性機制研究。大腦的可塑性調節是神經迴路因應內、外在的改變,而產生的構造與功能上的調整,因此可塑性調節是被認為大腦訊息儲存的細胞機轉。最近我們將研究的目標轉移到大腦中,與痛覺的處理、認知調控有關的區域,包括杏仁核區與視丘。我們希望在藉由探討慢性痛動物模式中,這些區域所可能產生的突觸傳導、可塑性與神經細胞興奮性的功能改變為觀點,來詮釋慢性痛的痛敏感化產生的分子與細胞機制。

Research

  1. Synaptic plasticity in thalamus and hippocampus.
  2. Analysis of physiological and morphological characteristics in Neurons of A7 Catecholamine cell group and trigeminal premotor neurons.

Laboratory

The research interests in this Lab include, pain mechanism, synaptic plasticity in thalamic neurons, and neuronal control of cardiovascular functions. For pain mechanism study, we have successfully made whole cell patch clamp recording from a group of NE (norepinephrine) releasing neurons in brainstem (the A7 Catecholamine cell group). These neurons project their nerve terminals to dorsal horn of spinal cord, and release NE that directly inhibit nociceptive signal. We have already characterized the firing pastern and other basic physiological properties of these neurons, and also recently demonstrated that substance P (SP), a well-known neuropeptide with potent antinociceptive effect, could dramatically increase excitability by opening TRPC channels in these NE releasing neurons.

For the study of synaptic plasticity in thalamic neurons, we are interested in spiking timing-dependent plasticity in the thalamic neurons. We have found that long-term potentiation (LTP) of synaptic transmission could be induced in thalamic neurons, and continue to investigate if the different spiking mode had any effect on LTP in thalamic neurons. We are also interested in exploring the effect of anesthetics on motoneurons controlling cardiovascular functions, a key issues in anesthesiology. We make whole cell recording from motoneruons involved in cardiovascular function, and test the effect of some generally used anesthetics on the excitability and synaptic transmission in these motoneurons.

代表著作 (最近五年)

Publications (Recent 5 years)

Journal
  1. Chen, C.-C., Shen, J.-W., Chung, N.-C., Min, M.-Y., Cheng, S.-J., Liu, I.Y.(2012) Retrieval of context-associated memory is dependent on the Ca v3.2 T-type calcium channel. PLoS ONE, 7 (1), art. no. e29384, .
  2. Min, M.-Y., Yang, H.-W., Yen, C.-T., Chen, C.-C., Chen, C.-C., Cheng, S.-J.(2011) ERK, synaptic plasticity and acid-induced muscle pain. Communicative and Integrative Biology, 4 (4), pp. 394-397.
  3. Wu, Y., Wang, H.-Y., Lin, C.-C., Lu, H.-C., Cheng, S.-J., Chen, C.-C., Yang, H.-W., Min, M.-Y.(2011) GABA b receptor-mediated tonic inhibition of noradrenergic A7 neurons in the rat. Journal of Neurophysiology, 105 (6), pp. 2715-2728.
  4. Cheng S-J, Chen C-C, Yang H-W, Chang Y-T, Bai S-W, Chen C-C, Yen C-T, Min M-Y*. (2011) Role of extracellular signal-regulated kinase in synaptic transmission and plasticity of a nociceptive input on capsular central amygdaloid neurons in normal and acid-induced muscle pain mice. Journal of Neuroscience, 31 (6), pp. 2258-2270.
  5. Hsu J-C, Cheng S-J, Yang H-W, Wang H-J, Chiu T-H, Min M-Y, Lin Y-W. (2011) Bi-directional synaptic plasticity induced by conditioned stimulations with different number of pulse at hippocampal CA1 synapses: roles of N-methyl-D- aspartate and metabotropic glutamate receptors. Synapse, 65 (8), pp. 795-803.
  6. Hsu C-L, Yang H-W, Yen C-T, Min M-Y. (2010) Comparison of synaptic transmission and plasticity between sensory and cortical synapses on relay neurons in the ventrobasal nucleus of the rat thalamus. J Physiol (London) 588: 4347-4363. (Selected and evaluated by Faculty 1000 in Biology and Medicine)
  7. Min M-Y, Wu Y-W, Shih P-Y, Lu H-W, Wu Y, Hsu C-L, Li M-J, Yang HW. (2010) Roles of A-type potassium currents in tuning spike frequency and integrating synaptic transmission in noradrenergic neurons of the A7 catecholamine cell group in rats. Neuroscience 168:633-645.
  8. Wu WL, Lin YW, Min MY, Chen CC (2010) Mice lacking Asic3 show reduced anxiety-like behavior on the elevated plus maze and reduced aggression. Genes Brain Behav 9:603-614.
  9. Min M-Y, Shih P-Y, Wu Y-W, Lu H-W, Lee M-L, Yang H-W et al. (2009) Neurokinin 1 receptor activates transient receptor potential-like currents in noradrenergic A7 neurons in rats. Mol Cell Neurosci 42:56-65.
  10. Min M-Y, Wu Y-W, Shih P-Y, Lu H-W, Lin C-C, Wu Y, Li M-J, Yang H-W (2008) Physiological and morphological properties of, and effect of Substance P on, neurons in the A7 catecholamine cell group in rats. Neuroscience 153:1020-1033.
  11. Lin Y-W, Min M-Y, Lin C-C, Chen W-N, Wu W-L, Yu H-M, *Chen C-C. (2008) Identification and characterization of a subset of mouse sensory neurons that express acid-sensing ion channel 3. Neuroscience 151:544-557.
  12. Lin Y-W, Yang H-W, Min M-Y, Chiu T-H. (2008) Inhibition of associative long- term depression by activation of beta-adrenergic receptors in rat hippocampal CA1 synapses. J Biomed Sci 15:123-131.
  13. Yang S-C, Chiu T-H, Yang H-W, Min MY. (2007) Presynaptic adenosine A1 receptors modulate excitatory synaptic transmission in the posterior piriform cortex in rats. Brain Res. 1156:67-79.
  14. Min M-Y, Hsu P-C, Lu H-W, Lin C-J, Yang H-W. (2007) Postnantal development of noradrenergic terminals in rat trigeminal motor nucleus: a light and electron microscopic immunocytochemical analysis. Anat Rec 290: 96-107.
  15. Lin Y-W, Yang H-W, Chiu T-H, Min M-Y. (2006) Spike-timing dependent plasticity at resting and conditioned lateral perforant path synapses on granule cells in the dentate gyrus: different roles of N-methyl-d-aspartate and group I metabotropic glutamate receptors. Eur J Neurosci 23: 2362-2374.

Conference
  1. Min M-Y, Cheng S-J, Chen C-C , Chen C-C, Yang H-W. (2010) Effect of extracellular signal-regulated kinases on synaptic transmission and plasticity between parabrachial nucleus and capsular division of central amygdala in normal and chronic muscle pain mice. FENS Abstr., vol.5, 014.23, 2010.
  2. Lu C- C, Chen R-F, Min M-Y, Chen C-C. (2010) GABAA receptor mediated tonic inhibition in the A7 catecholamine cell group in rats. FENS Abstr., vol.5, 075.22, 2010.
  3. Chen H-H, Lu H-W, Min M-Y, Yang H-W. (201) Orexin-immunoreactive terminals in the rats A7 area: light- and electron-microscopic analysis of their relationships with noradrenergic neurons. FENS Abstr., vol.5, 111.5, 2010.
  4. Li M-J, Min M-Y, Yang H-W. (2010) Cholinergic modulation in A7 noradrenergic neurons. FENS Abstr., vol.5, 140.17, 2010.
  5. Ynag H-W, Wu Y, Min M-Y. (201) GABAB receptor mediated tonic inhibition in noradrenergic neurons of the A7 catecholamine cell group in rats. FENS Abstr., vol.5, 140.29, 2010.
  6. Kao H-Y, Min M-Y, Lai W-S (2010) Altered membrane and morphology properties in medium spiny neurons of dorsal striatum in Akt1 knock out mice. FENS Abstr., vol.5, 196.13, 2010.
  7. Hsu C-L, Yang H-W, Min M-Y. (2009) L-type calcium channel-dependent long-term depression (LTD) of cortical input on relay neurons in the ventrobasal nucleus of rat thalamus. Program No. 320.1/C51. 2009 Neuroscience Meeting Planner. Chicago, IL: Society for Neuroscience, 2009. Online.
  8. Chiang Y-H, Cheng S-J, Yang F-C, Min M-Y, Wen C-C, Hwu H-G, Fu W-M. (2009) Role of schizophrenia-related gene of neuregulin 1 in fear memory and depression. Program No. 248.13/N12 2009 Neuroscience Meeting Planner. Chicago, IL: Society for Neuroscience, 2009. Online.
  9. Min M-Y, Lin C-C, Yang H-W. (2008) GABA-B receptor opens GIRK channel in noradrenergic neurons of A7 catecholamine cell group in rats. FENS Abstr., vol.4, 043.14, 2008.
  10. Yang H-W, Lu H-W, Wu Y, Min M-Y (2008) Orexin-A depolarizes noradrenergic neurons through activation of transient receptor potential (TRP) V1 channels in A7 catecholamine cell group. FENS Abstr., vol.4, 180.40, 2008.
  11. Wu Y, Lin C-C , Min M-Y, Yang H-W (2008) GABAB receptor mediated tonic inhibition in noradrenergic neurons in A7 catecholamine cell group. Program No. 72.13/LL17. 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online
  12. Lee M-L, Yang H-W, Min M-Y (2008) Identification of synaptic connection between pontine parabrachail area and A7 catecholamine cell group in rats. Program No. 72.10/LL14. 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online.
  13. Chen R-F, Lee C-W, Min M-Y (2008) Pentobarbital acting at GABAA receptor induces a tonic current in cardiac vagal neurons in nucleus ambiguous. Program No. 580.22/RR8. 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online
  14. Cheng S-J, Li M-J, Yang H-W, Min M-Y (2008) Activation of extracellular signal-regulated kinase induces a long-term potentiation of synaptic transmission in parabrachio-amygdaloid pain pathway.` Program No. 368.3/JJ28. 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online
  15. Hsu C-L, Cheng S-J, Yang H-W, Min M-Y (2008) Synaptic transmission and plasticity of cortical and sensory inputs on relay neurons in the ventrobasal nucleus of rat thalamus. Program No. 39.1/K3 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online.
  16. Lu H-W, Min M-Y, Yang H-W (2007) Effect of orexin-a on inhibitory postsynaptic current and membrane excitability in noradrenergic neurons of a7 catecholamine cell group. Program No. 462.19/G15. 2007 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2007. Online.
  17. Hsu C-L, Cheng S-J, Yang H-W, Min M-Y (2007) Synaptic plasticity of sensory input on the replay neurons in ventrobasal nucleus of thalamus. Program No.47.11/K16. 2007 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2007. Online.
  18. Wu Y-W, Shih P-Y, Min M-Y, Yang H-W (2006) A role for Kv4.2 K+ channel in firing property of neurons in A7 catecholamine cell group in rats. Program No. 247.14/Q11. 2006 Neuroscience Meeting Planner. Atlanta, GA: Society for Neuroscience, 2006. Online
  19. Shih P-Y, Wu Y-W, Yang H-W, Min M-Y (2006) Substance P acting on NK1 receptor increases excitability of neurons in A7 catecholamine cell group in rats. Program No. 247.15/Q12. 2006 Neuroscience Meeting Planner. Atlanta, GA: Society for Neuroscience, 2006. Online

學術專書 Literature
  1. Min M-Y, Yang H-W, Lin Y-W (2008) Role of the noradrenergic system in synaptic plasticity in the hippocampus. In “Novel Trends in Brain Science” (M. Onozuka and C.-T. Yen Eds), pp149-164. Springer.

開設課程

  • 普通動物學
  • 普通生物學
  • 動物生理學(與嚴震東、陳瑞芬合開)
  • 神經生物學(與嚴震東、潘建源合開)
  • 神經生物學實驗